Figure 1
CILP1 expression in human cardiac tissue. (A) Increased CILP expression in human myocardial infarct tissue. CILP1 protein was detected in human myocardial tissue by Western blot analysis. RA; remote area tissue, MI; myocardial infarct tissue. Note that the tissue samples from RA and MI were obtained from the same human subject. Cartilage was included as a positive control for CILP1. (B) Western blot of endomyocardial biopsies from patients with aortic valve stenosis and control subjects. Lane 1, Control subject spiked with 1 ng recombinant CILP1; Lanes 2–7 Aortic valve stenosis (AS) patients; Lanes 8–10, control subjects. (C) Quantification of the CILP1 signal from the Western blot shown in B. The signal of CILP1 was corrected for the corresponding GAPDH signal. CILP1 protein levels are shown as mean ± SEM, relative to control subjects. (D) Gene expression analysis of cardiac biopsies from human aortic valve stenosis patients (n = 11) and patients undergoing CABG (n = 6). Pearson correlations were calculated between CILP1-expression and ejection fraction (%), and the expression of the following genes: MYH6, TGFB3, COL1A1, COL1A2, COL3A1, TSP2, BGN and MGP. All expression levels are expressed as normalized intensities.
