Figure 3
CILP1 expression levels in isolated cardiac cells. (A) CILP1 mRNA levels in adult rat cardiomyocytes (CMC) and cardiac fibroblasts (CFB) (n = 8–14 isolations). (B) CILP1 mRNA levels in neonatal rat cardiomyocytes (CMC) and cardiac fibroblasts (CFB) (n = 8–14 isolations). (C) CILP1 and (D) Col1A1 expression during isolation of adult rat cardiac fibroblasts; ARCT: adult rat cardiac tissue (n = 3), ARCC: adult rat cardiac cells (after collagenase, but before adhesion to culture flasks, n = 4), ARCF: adult rat cardiac fibroblasts (immediately after 1 hour attachment to culture flasks, n = 3). (E and F) Effect of serum-starvation and TGFβ1 on ACTA2 and CILP1expression levels in adult rat cardiac ventricular fibroblasts (n = 5 separate experiments). (G) Effect of TGFβ type 1 receptor inhibitor SB431542 for 24 hrs on adult rat cardiac fibroblast CILP1 expression levels in the presence of serum (n = 4). Data were normalized to the housekeeping gene Cyclophilin-A using the comparative threshold cycle (Ct) method by calculating 2ΔCt and multiplied by 1000 (formula 1000 * 2ΔCt), to enhance readability. Data are expressed as mean ± SEM. *p < 0.05; **p < 0.01.
