Figure 5
From: RNA metabolism is the primary target of formamide in vivo
Genome-wide sensitivity screening. (a) Non-essential gene deletion collection (Bioneer V5) was arrayed over nine plates in 384-spot density format and copied in YES reference (upper left panel) media as well as in YES-Formamide (2% v/v) (upper right panel) and incubated at 30° for two days. High resolution images were obtained and analysed by computer automated processing to detect defective growth in the presence of formamide. Upper central panels illustrate a random example where a sensitive deletion (white squares) is enlarged in the middle. (b) Kernel density plotting for two independent biological repeats of the high-throughput screen. Dotted lines indicate the cut-off used to be considered sensitive. (c) Colony size dot plot. Ratio between plate-standardized spot size of each deletion in the two independent biological repeats of the screen (Pearson correlation coefficient = 0.23). Dotted lines represent sensitivity cut-off for both repeats. (d) Venn diagram from two biological repeats to identify consistently sensitive candidates. (e) Direct hierarchical relationship within the Gene Ontology terms enriched in our study. Boxes include GO term and code, fold enrichment and its associated p-value from AnGeLi analysis. Note that enrichment becomes significant from the most specific (RNA metabolism genes) to the most general GO term (cellular macromolecule metabolic process).
