Table 1 Formamide-sensitive deleted loci.

From: RNA metabolism is the primary target of formamide in vivo

Systematic Gene I.D.

Gene Standard Name

Product

Characterisation Status

Sensitivity score Replicate I

Sensitivity score Replicate II

Human orthologue

SPAC13F5.07c

hpz2

zf PARP type zinc finger protein Hpz2

conserved unknown

0,000

0,000

 

SPAC15E1.03

rpl42

60S ribosomal protein L36/L42

published

0,230

0,026

RPL36AL/RPL36A

SPAC17G8.05

med20

mediator complex subunit Med20

published

0,038

0,022

MED20

SPAC1F7.13c

rpl801

60S ribosomal protein L8 (predicted)

biological role inferred

0,273

0,005

RPL8

SPAC20H4.02

dsc3

Golgi Dsc E3 ligase complex subunit Dsc3

published

0,254

0,012

 

SPAC22A12.07c

ogm1

protein O-mannosyltransferase Ogm1

published

0,005

0,155

POMT2

SPAC22F8.12c

shf1

small histone ubiquitination factor Shf1

published

0,293

0,214

PPHLN1

SPAC23H3.13c

gpa2

heterotrimeric G protein alpha-2 subunit Gpa2

published

0,060

0,100

 

SPAC27D7.14c

tpr1

RNA polymerase II associated Paf1 complex subunit Tpr1

published

0,102

0,046

CTR9

SPAC30D11.13

hus5

SUMO conjugating enzyme E2 Hus5

published

0,242

0,343

UBE2I

SPAC31 A2.06

atp25

mitochondrial ATP synthase complex assembly protein Atp25 (predicted)

biological role inferred

0,390

0,296

 

SPAC31G5.18c

sde2

silencing defective protein Sde2

published

0,339

0,230

SDE2

SPAC3G9.08ING5

png1

ING family homolog Png1

published

0,322

0,009

ING2/ING3/ING4/

SPAC3H8.05c

mms1

E3 ubiquitin ligase complex subunit Mms1 (predicted)

biological role inferred

0,005

0,000

 

SPAC4F10.06

bud22

ribosome small subunit biogenesis protein, BUD22 family (predicted)

biological role inferred

0,217

0,000

SRFBP1

SPAC869.03c

SPAC869.03c

urea transmembrane transporter (predicted)

biological role inferred

0,262

0,304

 

SPAC8C9.07

fyv7

rRNA processing protein Fyv7 (predicted)

biological role inferred

0,000

0,000

CCDC59

SPAC9.13c

cwf16

splicing factor Cwf16

published

0,296

0,119

CCDC94

SPAPB1E7.02c

mcl1

DNA polymerase alpha accessory factor Mcl1

published

0,243

0,000

WDHD1

SPBC16H5.03c

uba2

SUMO activating enzyme E1-type Uba2 (predicted)

biological role inferred

0,337

0,173

UBA2

SPBC1718.03

ker1

DNA-directed RNA polymerase I complex subunit Ker1

published

0,042

0,006

 

SPBC17A3.05c

SPBC17A3.05c

DNAJ/DUF1977 DNAJB12 homolog (predicted)

biological role inferred

0,019

0,001

DNAJB14/DNAJB12

SPBC19G7.10c

pdc2

topoisomerase II-associated deadenylation-dependent mRNA-decapping factor Pdc2 (predicted)

published

0,216

0,179

PATL1

SPBC24C6.11

cwf14

G10 protein

published

0,046

0,004

BUD31

SPBC32F12.05c

cwf12

complexed with Cdc5 protein Cwf12

published

0,042

0,175

ISY1

SPBC337.03

rhn1

RNA polymerase II transcription termination factor homolog

published

0,365

0,287

RPRD1A/RPRD1B

SPBC365.06

pmt3

SUMO

published

0,260

0,013

SUMO1/SUMO1P1

SPBC3D6.08c

lsm1

mRNA decapping complex subunit (predicted)

biological role inferred

0,221

0,000

LSM1

SPBC83.12

SPBC83.12

Schizosaccharomyces pombe specific protein

uncharacterized

0,003

0,211

 

SPBC9B6.07

nop52

nucleolar protein Nop52 family Rrp1 (predicted)

biological role inferred

0,359

0,157

RRP1/RRP1B

SPBP23A10.16

sdh4

TIM22 inner membrane protein import complex anchor subunit Tim18

published

0,000

0,038

SDHD

SPCC1739.14

npp106

nucleoporin Npp106

published

0,000

0,000

NUP93

SPCC297.03

ssp1

Ca2+/calmodulin-dependent (CaMMK)-like protein kinase Ssp1

published

0,228

0,144

CAMKK1/CAMKK2

SPCC338.16

pof3

F-box protein Pof3

published

0,353

0,001

STIP1

SPCC4B3.10c

ipk1

inositol 1,3,4,5,6-pentakisphosphate (IP5) kinase

published

0,002

0,000

IPPK

SPCC794.12c

mae2

malic enzyme, malate dehydrogenase (oxaloacetate decarboxylating), Mae2

published

0,019

0,033

ME1/ME2/ME3

  1. Non-essential loci deletions that meet specified criteria in both biological repeats to be considered formamide sensitive (see Materials and Methods). 80% of them have at least one manually curated human orthologue in PomBase42. “Sensitivity score” corresponds to the spot size ratio between control and experiment plates after normalization. Bold font rows denote genes which belong to significantly enriched GO terms found in this study (indicated in Fig. 5e).