Figure 5
FRET-enhanced intensity ratio between the Lo and Ld domains. Confocal fluorescent images of SM/POPC/chol (in a mole ratio of 1:1.2:0.5) GUVs in the presence of 0.2 mol% Bodipy-PC (a) and neg-SMs (b–d). The micrograph of GUVs (a) was different from those of (b–d), while the images (b–d) were obtained from the same GUVs. The Lo domains are indicated by curved arrows (d). The bar indicates 20 μm. (e–g) show the intensity profiles along the dashed lines in (b–d), respectively. Intensity profiles of (b–d) along the edge of the GUV were plotted in (e–g), respectively, from θ = 0° to 180°. The merged regions of the two GUVs (indicated by arrowheads in b–d) are not included in the angle range.
