Figure 6 | Scientific Reports

Figure 6

From: Emphatic visualization of sphingomyelin-rich domains by inter-lipid FRET imaging using fluorescent sphingomyelins

Figure 6

Single-cell confocal (a) monochromic, (b) FRET, and (c) their merged images of the apical membrane of cultured epithelial (EpH4) cells labeled with 488neg-SM and 594neg-SM. These images show the same region. The bar indicates 10 ÎĽm. Green and red profiles in (d) represent the fluorescent intensity along the dashed lines in (a) and (b), respectively. Arrows indicate peaks with intense monochromic signals, but weak or no FRET, and an asterisk indicates a FRET signal with weak monochromic peak.

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