Figure 3 | Scientific Reports

Figure 3

From: A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes

Figure 3

Results of pore-free island recognition and Nups foci detection. (a) The first row shows the mAb414-staining images with aphidicolin or roscovitine for 6 or 10 h; the second row shows the results of pore-free island recognition. The red lines indicate the boundary between the pore-rich region and the pore-free island. The third row presents POM121-staining images, showing the region with pore-free island recognised in the mAb414-staining images with red lines. The fourth row shows the automatic detection results of POM121 foci within each pore-free island. Representative POM121 foci are indicated by the green points. Scale bar, 5 μm. (b)The first and second rows show the enlarged image sets of the mAb414-staining images, and results pertaining to the recognition of pore-free islands are shown in Fig. 3a. The third and fourth rows show the enlarged image sets of Nup-foci images, and the results pertaining to the detection of POM121 foci are shown in Fig. 3a.

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