Figure 6 | Scientific Reports

Figure 6

From: A statistical image analysis framework for pore-free islands derived from heterogeneity distribution of nuclear pore complexes

Figure 6

Comparison of appearance frequency between observed and simulated Nup foci. (a) Schematic illustration of the distance measurement from each Nup focus to the boundary between pore-free islands and pore-rich regions. The shortest distances of each Nup focus detected in Fig. 5 were measured by distance transformation and the appearance frequencies in each distance were calculated (observed data). The observed data were compared with the data simulated using actual locations of pore-free islands and the number of Nups foci detected in each pore-free island (simulated data). (b) Positions of Nup foci with respect to NUP93, NUP107, or POM121 in pore-free islands were quantified in cells treated with aphidicolin or roscovitine for 6 or 10 h using the experimental real data. The quantified appearance frequencies were averaged and normalised, and then plotted for each experimental condition. Green and blue lines show the observed and simulated data, respectively.

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