Table 7 PCR primers, restriction enzymes and diagnostic DNA fragments used in the experiment.
No. | Target gene | Primer sequence (5′-3′) | DNA fragment (bp) | Restriction enzyme | |
|---|---|---|---|---|---|
Forward | Reverse | ||||
1 | GSTM1 (−/+)a | CTCCTGATTATGACAGAAGCC | CTGGATTGTAGCAGATCATGG | 650 | — |
2 | GSTT1 (−/+)a | TTCCTTACTGGTCCTCACATCTC | TCACCGGATCATGGCCAGCA | 480 | — |
3 | β-Globulina | CAACTTCATCCACGTTCACC | GAAGAGCCAAGGACAGGTAC | 268 | — |
4 | GSTA1 (C69T)b | TGTTGATTGTTTGCCTGAAATT | GTTAAACGCTGTCACCCGTCCT | 480 (380/100) | EarI |
5 | GSTP1 (A1578G)b | ACCCCAGGGCTCTATGGGAA | TGAGGGCACAAGAAGCCCCT | 176 (91/85) | BsmAI |
6 | CYP2E1 (C1019T)b | TTCATTCTGTCTTCTAACTGG | CCAGTCGAGTCTACATTGTCA | 410 (360/50) | RsaI |
7 | CYP3A4 (A13871G)b | CACATTTTCTACAACCATGGAGACC | GAATCTGGTGGGGACAGGTATAAA | 249 (14/141/94) | BsmAI |
8 | SLCO1B1 (T521C)b | TTGTCAAAGTTTGCAA AGTG | GAAGCATATTACCCATGAGC | 209 (189/20) | Hha I |
9 | SLCO1B3 (T334G)b | GAAGGTACAATGTCTTGGGC | CTCTCAAAAGGTAACTGCC | 253 (213/40) | Alu I |
