Figure 4
From: Alanine substitution in cellobiohydrolase provides new insights into substrate threading
Hydrolytic activity assays for N62A mutant. (a) Chromatograms of reaction aliquots with N62A and cellobiose standard. Upper two charts show the retention time ranges 0–15 min and 7.8–8.5 min. Black line: reaction aliquot with substrate. Grey line: reaction aliquot without substrate. Third chart shows the difference in the two values (with and without substrate). Fourth chart shows the chromatogram of cellobiose, 0.15% (w/v). (b) Hydrolytic activity of WT at three different concentrations and the N62A mutant. Cellobiose refers to the HPLC peak area of cellobiose delivered by the action of TcCel7A in the activity assay. Black circles: WT. Blue diamond: N62A mutant, assuming a concentration below 0.5 mg/L in the reaction mixture. Error bar: Standard deviation.
