Table 2 Hydrolytic activities of WT, N62A, and K203A from different culture batches on different substrates. Hydrolytic activity values from duplicated experiments are listed with standard deviations in parentheses.
From: Alanine substitution in cellobiohydrolase provides new insights into substrate threading
Substrate | Assay | Activity | Culture no. of N62A | ||
|---|---|---|---|---|---|
WT | N62A | K203A | |||
Cellulose, Sigma (excerpted from Table 1) | HPLC | 1.00 (0.01) | >3.85 (0.49) | 1.26 (0.05) | 1 |
Cellulose, Merck | HPLC | 1.00 (0.04) | >4.57 (0.45) | 1.36 (0.05) | |
Cellulose, Merck | HPLC | 1.00 (0.05) | >3.78 (0.90) | ND | 2 |
Cellulose, Sigma | DNS | 1.00 (0.42) | 2.20 (1.12) | 1.89 (0.58) | 3 |
Pretreated corn stover | HPLC | 1.00 (0.07) | >3.92 (0.34) | 1.00 (0.15) | 4 |
