Figure 2
Expression of the key metabolic regulators in the livers of NC- or HFD- fed mice exposed to PM2.5 or FA. (A) Western blot analyses of levels of PPARγ, PPARα, SIRT1, and ApoA4 in the liver tissues from NC- or HFD- fed mice exposed to PM2.5 or FA for 10 weeks. Levels of β-actin were determined as loading controls. (B) Quantitative analyses of fold changes of PPARγ, PPARα, SIRT1, and ApoA4 protein levels, as determined by Western blot densitometry, in the livers of NC- or HFD- fed mice exposed to PM2.5 or FA. Each bar denotes mean ± SD. *p < 0.05. (C) Expression levels of Sirt1, Pparα, Pparγ, Apoa4, and Fasn mRNAs in the livers of NC- or HFD- fed mice exposed to PM2.5 or FA for 10 weeks. Expression levels of mRNAs were determined by qPCR. Fold changes of mRNA levels are shown by comparing to that of one of NC-fed mice under FA exposure. Each bar denotes mean ± SEM (n = 4 mice per group). *p < 0.05. Fasn, fatty acid synthase.
