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Figure 1

From: Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity

Figure 1

Acetaminophen activates hepatic Nrf2 signalling in vivo. Wild type C57Bl/6 J mice (n = 5 per group) were administered saline or 300 mg/kg APAP. (A) Total hepatic GSH content and (B) serum ALT levels in mice at the indicated times post-APAP administration. (C) qPCR analysis of Nrf2 target genes in the livers of mice at the indicated times. (D) Immunoblot analysis of Hmox1 and Nqo1 in livers of mice (two representative samples per group) at the indicated times. (E) Densitometric analysis of Hmox1 and Nqo1 protein levels in livers of mice (five per group) at the indicated times. Data represent mean + S.D. Statistical analysis was performed with (A) one-way ANOVA (Tukey’s multiple comparison) or (B,C,E) a Kruskal-Wallis (Conover-Inman pairwise comparison) test; *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001 versus 0 h. @P ≤ 0.001 versus 6 h saline.

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