Figure 2 | Scientific Reports

Figure 2

From: Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity

Figure 2

Acetaminophen provokes a localised hepatic Nrf2 stress response. Nrf2-luc mice (n = 5 per group) were administered saline or 300 mg/kg APAP. (A) In vivo bioluminescence imaging of the same representative mice at the indicated times post-APAP administration. See Fig. S2 for imaging data for all mice. (B) Ex vivo bioluminescence imaging of livers and kidneys of the mice shown in A, 24 h post-APAP administration. (C) Luminescence signals from in vivo and ex vivo imaging of all mice. (D) Correlation of bioluminescence signals in livers imaged ex vivo and serum ALT levels in the same mice. (E) qPCR analysis of Nrf2 target genes in the livers of mice 24 h post-APAP administration. (F) Immunoblot analysis of Hmox1 in livers of mice at 24 h. Data represent mean + S.D. Statistical analysis was performed with a (C) Mann-Whitney U test, (D) Pearson’s R test or (E) unpaired t-test; *P ≤ 0.05, **P ≤ 0.001, ***P ≤ 0.0001 versus saline.

Back to article page