Figure 4 | Scientific Reports

Figure 4

From: Real-time in vivo imaging reveals localised Nrf2 stress responses associated with direct and metabolism-dependent drug toxicity

Figure 4

Cisplatin provokes a localised renal Nrf2 stress response. Nrf2-luc mice (n = 4 per group) were administered saline or 20 mg/kg cisplatin. (A) In vivo bioluminescence imaging of the same representative mice at the indicated times post-cisplatin administration. See Fig. S5 for imaging data for all mice. (B) Ex vivo bioluminescence imaging of kidneys and livers of the mice shown in A, 96 h post-cisplatin administration. (C) Luminescence signals from in vivo and ex vivo imaging of all mice. (D) Correlation of bioluminescence signals in kidneys imaged ex vivo and serum BUN levels in the same mice. (E) Immunoblot analysis of Hmox1 in kidneys of mice at 96 h. Data represent mean + S.D. Statistical analysis was performed with a (C) Mann-Whitney U test or (D) Pearson’s R test; *P ≤ 0.05 versus saline.

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