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Figure 1

From: Baculovirus-Mediated miR-214 Knockdown Shifts Osteoporotic ASCs Differentiation and Improves Osteoporotic Bone Defects Repair

Figure 1

miR-214 regulates the switching of adipogenesis and osteogenesis in OVX-ASCs. OVX-ASCs were mock-transduced (Mock group) or co-transduced with BacECre/Bac214S at MOI 100/150 (214 S group). ASCs isolated from Sham animals (Sham) served as a control. (a) qRT-PCR analysis of miR-214. The cells were harvested at 2 days post-transduction (dpt) for analysis. (b) qRT-PCR analysis of marker genes in early (runx2) and middle (ocn) stage of osteogenic differentiation at 4 and 10 dpt, respectively. (c) qRT-PCR analysis of adipogenic marker genes (ppar-γ and c/ebp-α) at 4 dpt. (d) Alizarin red and AdipoRed staining performed at 14 dpt. Alizarin red stained calcium and hence mineralization. AdipoRedTM stained intracellular lipid droplets and cells committing to adipogenic lineage emitted green fluorescence under the confocal microscope. The percentages of cells emitting fluorescence are shown.

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