Figure 2 | Scientific Reports

Figure 2

From: Baculovirus-Mediated miR-214 Knockdown Shifts Osteoporotic ASCs Differentiation and Improves Osteoporotic Bone Defects Repair

Figure 2

miR-214 targeted TAB2 and CTNNB1 in the Wnt pathway to switch osteogenesis/adipogenesis. (a) Reporter plasmids expressing Gluc and Fluc, with wild-type or mutant TAB2 (TAB2-wt or TAB2-mut) sequences at the 3′ UTR of Fluc. (b) Reporter plasmids expressing Gluc and Fluc, with wild-type or mutant CTNNB1 (CTNNB1-wt or CTNNB1-mut) sequences at the 3′ UTR of Fluc. (c) Relative luciferase activities in cells transfected with TAB2-wt or TAB2-mut. (d) Relative luciferase activities in cells transfected with CTNNB1-wt or CTNNB1-mut. (e) Western blot analysis. (f) Densitometry analysis of bands in Western blot. Sham-ASCs and OVX-ASCs were mock-transduced (Sham-Mock and OVX-Mock) or co-transduced with BacECre/Bac214S (Sham-214S and OVX-214S). Cells were transfected with one of these 4 plasmids, followed by measurement of Fluc and Gluc activities 3 days later. Transfection efficiency was calibrated by Gluc activity and Fluc/Gluc were normalized to those in the Sham-Mock or Sham-214S groups to yield relative luciferase activities.

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