Figure 3 | Scientific Reports

Figure 3

From: Baculovirus-Mediated miR-214 Knockdown Shifts Osteoporotic ASCs Differentiation and Improves Osteoporotic Bone Defects Repair

Figure 3

Suppressing miR-214 in OVX-ASCs stimulated the osteogenesis of co-cultured OVX-BMSCs. (a) Co-culture of ASCs (Sham or OVX) with OVX-BMSCs in transwell assays and Alizarin red staining. (b) qRT-PCR analysis of osteogenic genes. Sham-ASCs (Sham), mock-transduced OVX-ASCs (Mock) and BacECre/Bac214S-transduced OVX-ASCs (214S) were seeded to transwell inserts, while OVX-BMSCs were seeded to the bottom of transwell plates. The cells were co-cultured in osteogenic medium for 15 days. OVX-BMSCs were stained by Alizarin red at 15 dpt and analyzed by qRT-PCR for osteogenic gene expression.

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