Figure 6 | Scientific Reports

Figure 6

From: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Figure 6

Effect of coexpression of helper genes on PLA2 production by K. phaffii induced by methanol in shake flasks. The strains harboring 12-copy (K. phaffii GS115/pPIC9K-PLA2-12) and 1-copy (K. phaffii GS115/pPIC9K-PLA2-1) PLA 2 gene were coexpressed with helper genes (CPR6, FES1, STI1, and SSA1), respectively. Coexpression of Ssa1 was used as positive control. K. phaffii GS115/pPIC9K-PLA2-12/pPICZ and K. phaffii GS115/pPIC9K-PLA2-1/pPICZ were used as negative control, respectively. Error bars indicate the standard deviation of three biological replicates. (A,B) Cell growth profiles; (C,D) Total protein concentration in the supernatant; (E) SDS-PAGE of total protein in the supernatant; S, M and “Control” indicate the 1-copy strain, the 12-copy strain and the negative control, respectively.

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