Table 1 Sample information for RNA-Seq&.

From: Identification of novel factors enhancing recombinant protein production in multi-copy Komagataella phaffii based on transcriptomic analysis of overexpression effects

Sample name*

Strain/plasmid

PLA 2 gene copy number

PLA2 protein concentration (g L−1)#

PLA2 Enzyme activity (U mL−1)

Relative PLA 2 transcription level

µ (h−1)

qs (gmethanol gDCW −1 h−1)

qp (U gDCW −1 h−1)

MC

GS115/pPIC9K-PLA2-12

12

5.7 ± 0.4

720 ± 34

4.5 ± 0.3

0.015 ± 0.003

0.17 ± 0.02

0.11 ± 0.01

SC

GS115/pPIC9K-PLA2-1

1

1.1 ± 0.3

115 ± 20

1.0 ± 0.2

0.031 ± 0.005

0.14 ± 0.02

0.04 ± 0.01

BK

GS115/pPIC9K

0

—

—

—

0.029 ± 0.004

0.12 ± 0.02

—

  1. *Sampling at 48 h after methanol induction in fed-batch fermentation.
  2. &Data are the mean and standard deviation of three biological replicates. Two biological replicates of each sample were used for RNA-Seq. µ: specific growth rate; qs: specific substrate (methanol) consumption rate; qp: specific product formation rate; DCW: dry cell weight; µ, qs and qp were calculated during the methanol induction time from 0 to 48 h.
  3. #The total protein concentration in the supernatant was determined by the Bradford method, and then the PLA 2 concentration was calculated according to the percentage of PLA 2 on the SDS-PAGE by the Quantity One software.
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