Figure 1
The production/activity of DctA depend on the presence of DauA. (A) Growth of the isogenic wild-type and ΔdctA, ΔdauA and ΔdctA/ΔdauA strains on MOPS minimal medium (pH 7) containing 50 mM of fumarate or glucose as sole carbon source. The plates were incubated for 24 hrs at 37 °C. (B) The wild-type (●),ΔdctA (▲),ΔdauA (■) and ΔdctA/ΔdauA (♦) strains grew in eM9 minimal medium at pH 7 containing 50 mM glucose as sole carbon source. Growth at 600 nm were monitored using microplates. All results are the averages of at least 4 independent test series. Error bars represent the standard deviation. (C) Same than B but the medium contained 50 mM fumarate as sole carbon source. (D) Time dependence of [14C]-fumarate accumulation in BW25113 (wild-type; ●), EK1 (ΔdauA; ■), EK2 (ΔdctA; ▲) and EK3 (ΔdctA/ΔdauA; ♦) strains. Measurements done after the cells were grew for 6 hrs in M9 containing 50 mM fumarate as the sole carbon source at pH 7. All results are the averages of at least 6 independent test series. Error bars represent the standard deviation.
