Figure 2 | Scientific Reports

Figure 2

From: The E. coli dicarboxylic acid transporters DauA act as a signal transducer by interacting with the DctA uptake system

Figure 2

The presence of DauA is necessary for optimal DctA production. (A) The wild-type (●),DauA-H6 (○), DctA-H6 (dashed line), DauA-H6ΔdctA (♦) and DctA-H6ΔdauA (■) strains were grown in eM9 minimal medium at pH 7 containing 50 mM fumarate as sole carbon source and the curves at 600 nm were recorded using microplates. All results are the averages of at least 4 independent test series. (B) Membrane fractions were prepared from DctA-H6, DctA-H6ΔdauA, DauA-H6, DauA-H6ΔdctA after growth for 6 hrs in M9 minimal medium supplemented with 50 mM fumarate as sole carbon source at pH 7. Samples were subjected to SDS-PAGE, followed by Western blotting with an anti-His antibody. The same membranes were subsequently stripped and re-hybridized with a polyclonal anti-TatC antibody as a control.

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