Figure 5

DauA and DctA form a complex. Chromosomally encoded C-terminally His-tagged DauA was purified by IMAC. Elution fractions were analyzed by Western immunoblotting using an anti-His antibody (top panel) and anti-FLAG antibody (bottom panel). The IMAC elution fractions were re-purified by FLAG affinity chromatography and analysed by Western blotting.