Table 2 Strains and plasmid use in this work.

E. coli K-12

WT (BW25113)

F-, Δ(araD-araB)567, ΔlacZ4787(::rrnB-3), λ-, rph-1, Δ(rhaD-rhaB)568, hsdR514

44

EK1

BW25113, but ΔdauA

4

EK2

BW25113, but ΔdctA

4

EK3

BW25113, but ΔdauA/ΔdctA

4

EK4

BW25113 encoding DauA-His¹

4

EK5

EK2 encoding DauA-His¹

4

EK6

BW25113 encoding Dcta-His¹

4

EK7

EK1 encoding DctA-His¹

4

EK9 IMW385

EK4 encoding DctA-FLAG¹ MC4100, but λ[Φ(dctA–lacZ)hyb Amp^R]

This study45

EK8

IMW385, but ΔdauA

4

pBluescript-II KS+

Amp^R

Stratagene

pMAK705

pSC101 derivative- rep(ts) - Cm^R

43

pMW2031

yfp(A206K)-dauA with N-terminal His6-Tag, pBAD30 derivative (Amp^R)