Figure 6
From: Identification of differentially expressed miRNAs in the fatty liver of Landes goose (Anser anser)
Validation of selected target genes. (a) Binding sites for aan-miR-203a in the ACSL1 3′UTR of different species; (b) Binding sites for aan-miR-125b-5p in the ELOVL6 3′UTR of different species; (c) Relative expression of ACSL1 and ELOVL6 in control and overfed liver samples; (d) Luciferase activity assay of the recombined Dual-Luciferase reporter vectors with 3′UTR of ACSL1 or ELOVL6 co-transfected with aan-miR-203a mimic or aan-miR-125b-5p mimic; (e) Relative expression of ACSL1 and ELOVL6 in hepatocytes transfected with aan-miR-203a mimic or aan-miR-125b-5p mimic; (f) The relative content of intracellular lipid in hepatocytes transfected with aan-miR-203a mimic or aan-miR-125b-5p mimic. **Means P < 0.01.
