Figure 8

Singlet oxygen production and chlorophyll photo-bleaching in isolated membranes. Panel a: singlet oxygen production indirectly determined following the increase of fluorescence of Singlet Oxygen Sensor Green (SOSG), a fluorescent probe increasing its fluorescence in presence of singlet oxygen. Isolated membranes were illuminated with red light at 6000 µmol m−2s−1. All data were normalized to chlorophyll content and to the SOSG fluorescence of green membranes after 30′ of illumination. Panel b: chlorophylls photo-bleaching induced in isolated membranes upon illumination with white light at 6000 µmol m−2s−1. Standard deviations are indicated in both panels (n = 3).