Table 1 Pigment analysis of isolated photosynthetic complexes. Pigment extracts were analyzed by HPLC. Pigment data from B2 and B3 fractions were normalized to 14 chlorophylls; pigments from B4 fractions were normalized to 72 chlorophylls; pigments from B5 fractions were normalized to 170 chlorophylls. Chl: chlorophyll; Car: carotenoid; Neo: neoxanthin; Viola: violaxanthin; Antera: anteraxanthin; Lute: Lutein; Zea: zeaxanthin; β-Car: β-carotene; Asta: astaxanthin; Asta ester: esterified forms of astaxanthin. Standard deviations are below 8% for each value reported in the table (n = 3).

From: Functional analysis of photosynthetic pigment binding complexes in the green alga Haematococcus pluvialis reveals distribution of astaxanthin in Photosystems

 

Chl

Chla/Chlb

Chl a

Chl b

Chl/Car

Neo

Viola

Asta

Antera

Lute

Zea

Asta ester

β-Car

GREEN B1

100

2.8

73.7

26.3

0.8

6.9

36.9

n.d.

n.d.

73.8

n.d.

n.d.

6.2

ORANGE B1

100

3.3

76.9

23.2

0.2

11.5

59.6

10.2

11.8

69.6

8.0

434.6

6.6

RED B1

100

4.2

80.9

19.1

0.1

12.0

105.2

17.8

23.1

91.4

14.7

1352.5

27.3

GREEN B2

14

1.6

8.6

5.4

4.0

0.7

0.7

n.d.

n.d.

2.7

n.d.

n.d.

n.d.

ORANGE B2

14

1.5

8.4

5.6

4.7

0.9

0.2

<0.1

n.d.

2.3

0.1

<0.1

<0.1

RED B2

14

1.5

8.4

5.6

4.5

0.9

0.2

<0.1

n.d.

2.4

0.1

0.1

<0.1

GREEN B3

14

1.7

8.8

5.2

3.8

0.7

0.8

n.d.

n.d.

2.8

n.d.

n.d.

n.d

ORANGE B3

14

1.5

8.5

5.5

4.8

0.8

0.3

<0.1

n.d.

2.3

0.1

n.d.

n.d.

RED B3

14

1.5

8.4

5.7

4.4

0.9

0.2

0.1

n.d.

2.3

0.1

0.1

n.d.

GREEN B4

72

22.6

68.9

3.1

4.3

0.3

1.0

n.d.

n.d.

3.3

n.d.

n.d.

12.1

ORANGE B4

72

29.4

69.6

2.4

6.3

0.6

0.6

0.6

n.d.

2.2

n.d.

1.9

5.4

GREEN B5

170

7.1

149.1

20.9

4.4

0.2

8.2

n.d.

n.d.

17.0

n.d.

n.d.

13.5

ORANGE B5

170

7.0

148.7

21.3

4.4

0.8

6.0

1.9

n.d.

18.0

1.0

0.9

10.6

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