Figure 7
Effect of crowding on heat stability of rC1-INH. 250 μg/ml C1-INH wild-type (wt, open symbols) and Arg378Cys (R378C, closed symbols) were incubated for 2 hours at various temperatures with (circles) or without (triangles) 30% Ficoll. Samples were centrifuged at 10,000 × g for 20 min, concentration of C1-INH antigen was measured by ELISA and expressed as the percentage of antigen concentration compared to samples kept at 4 °C. Each point was assessed at least in triplicate.
