Figure 8
Gel filtration of native and thermally denatured rC1-INH. 25 μl of 2 mg/ml wild-type rC1-INH (dashed line) and Arg378Cys mutant (solid line) were applied to a Superose6® column without pre-incubation (a), and with pre-incubation at 55 °C (b). The elution was continuously monitored at 280 nm. The arrows indicate the elution volume of marker proteins, from left to right: blue dextran 2,000, thyroglobulin (Mr 756 kd), ferritin (Mr 430 kd), catalase (Mr 213 kd).
