Figure 6
From: Estrogen receptor 1 (ESR1) regulates VEGFA in adipose tissue
Effect of E2 on ERs and HIF1A binding activity to the Vegfa gene promoter. 3T3-L1 adipocytes were treated with vehicle (0.01% DMSO; Control); 10 nM of E2 or 10 nmol/L of PPT for 24 hours. EMSA was performed as described in Materials and Methods. (a) ERs, biding in VEGFA promoter after 24 hours of E2 treatment. (b) HIF1A biding in VEGFA promoter after 24 hours of E2 treatment; (c) HIF1A binding in VEGFA gene promoter after 24 hours of PPT treatment. Representative images of the experiments are in the right. Results represent three independent experiments. Data are shown as the mean ± SEM of 4 to 6 samples. &P < 0.05 vs control.
