Figure 8
From: Estrogen receptor 1 (ESR1) regulates VEGFA in adipose tissue
VEGFA protein expression in gonadal (gWAT) and inguinal (iWAT) adipose tissue from WT and ERKO female mice. Immunofluorescence staining of VEGFA in gWAT (a) and iWAT (d). Nucleus in blue (DAPI), VEGFA in green, membrane marker in red, and composite in yellow/orange; arrows indicate VEGFA expression in adipocytes and arrowheads in vessels; scale 50 μm; Fluorescence intensity quantification in gWAT (b) iWAT (e). Adipocyte size (surface area- μm2) in gWAT (c) iWAT (f). Data on the graphs are means ± SEM of 4–7 samples, #p < 0.05 vs WT. NC = negative control.
