Figure 5
Effect of local sustained release of NGF (10 ng/ml) from hydrogels placed in front of the attached OVs. Local sustained release of NGF using hydrogel promoted axonal growth, stained by NFL in hESC- and hiPSC-derived RGCs ((b) and (f), respectively, magnification 40x), as compared with controls ((a) and (e), respectively). The shape of the filopodia of hESC- and hiPSC-derived RGCs ((d) and (h), respectively) was slightly promoted by NGF release, as compared with controls ((c) and (g), respectively), as evident from the staining of phalloidin and GAP43. The assessment from D24-D31 is performed in RMM. Each experiment was repeated at least five times. Scale bars in (a), (b), (e) and (f), 100 μm. Scale bars (c), (d), (g) and (h), 10 μm.
