Figure 7
Effect of locally sustained release of SLIT1 (5μg/ml) from hydrogels placed in front of the attached OVs. Locally sustained release of SLIT1 using hydrogel does not affect axonal growth of hESC- and hiPSC-derived RGCs ((a) and (d), respectively, magnification 40x). Only a subset of axons is repelled by SLIT1 release ((b) and (e), magnification 100x: squares in (a) and (d), respectively), while other axons remain unaffected. Filopodia of hESC- and hiPSC-derived RGCs ((c) and (f), respectively) have collapsed in response to SLIT1 release, as evident from phalloidin and GAP43 staining. The assessment from D27 is performed under RMM supplemented with 1.0% FBS and 100 ng/ml BDNF. Each experiment was repeated at least five times. Squares in (a) and (d) correspond to (b) and (e), respectively. Scale bars in (a) and (d), 100 μm. Scale bars in (b) and (e), 40 μm. Scale bars in (c) and (f), 10 μm.
