Figure 2

MBP immunodensity and MBP+ area in rodent models of neonatal brain injury. (a) Illustration of coronal section of rodent brain with the cortical locations (red squares) of obtained micrographs for the rat FIPH model. (b) Illustration of coronal section of rodent brain with cortical locations (red squares) of obtained micrographs for both mouse models of neonatal unilateral brain injury. (c) Representative pictures of MBP+ axons in the cortex of control rats (upper panels) and rats exposed to fetal inflammation (LPS at E18 + E19) and postnatal hypoxia (P4) (FIPH; lower panels). Left: original fluorescent signal of MBP staining (green); middle: grey-scale image of MBP fluorescent signal (white) from which immunodensity is measured; right: threshold set to include MBP+ signal (red) and exclude background to measure MBP+ area. (d,e) Bar graphs showing that rats exposed to FIPH display reduced MBP immunodensity as well as a decrease in MBP+ area (n = 8 per group). (f,i) MBP fluorescent signal (white) in the cortex of control mice exposed to sham-operation (upper panels) or mice exposed to HI + LPS at P5 (lower panel, f) or to HI at P9 (lower panel, i). (g) No change was observed in MBP immunodensity in mice exposed to HI + LPS at P5 compared to control mice (n = 6 per group). (h) P5 HI + LPS animals showed a decrease in MBP+ area compared to sham-operated controls (n = 6 per group). (j,k) Compared to controls, mice exposed to HI at P9 showed reduced MBP immunodensity (n = 8 per group) as well as a decrease in MBP+ area (n = 8 per group). Data represent mean ± SEM; *p < 0.05; **p < 0.01; ***p < 0.001.