Figure 6
From: A quantitative method for microstructural analysis of myelinated axons in the injured rodent brain
Segmentation of myelinated axons is also applicable on MBP-DAB staining and immunofluorescent PLP staining. (a) Contralateral hemisphere (upper panels) and ipsilateral hemisphere (lower panels) of mice exposed to unilateral HI at P9, at 2.5X (left) and 40X (middle) magnification. MBP-DAB staining allowed reliable segmentation and tracing of myelinated axons (right). (b) Contralateral hemisphere (upper panels) and ipsilateral hemisphere (lower panels) of mice exposed to unilateral HI at P9, at 2.5X (left) and 40X (middle) magnification. A fluorescent staining for myelin component PLP allowed segmentation and tracing of myelinated axons (right). Squares in 2.5X photograph represent the location of higher magnification images (middle).
