Figure 2 | Scientific Reports

Figure 2

From: A metagenome-derived thermostable β-glucanase with an unusual module architecture which defines the new glycoside hydrolase family GH148

Figure 2

Modular composition of EngU and an overview of EngU variants cloned in pETDuet. EngU fragments cloned in the first multiple cloning site (MCS1) are dark colored and written in square brackets while fragments in the second MCS2 are drawn as brighter bars. The pETDuet constructs were named according to the annotated parts of EngU, where A1 includes GH-A1, B is the CBM, A2 is GH_A2 and C includes the C-terminus with unknown function. The activities of successfully expressed proteins with the substrate barley β-glucan (percent relative to the full-length enzyme) are shown on the right. Derivatives marked with a star (*) were expressed as insoluble, inactive proteins.

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