Table 1 Purification of recombinant EngU. Activity was measured by the DNS assay with 0.5% barley β-glucan as the substrate in 50 mM McIlvaine buffer pH 6.0 after incubation for 10 min at 80 °C.

From: A metagenome-derived thermostable β-glucanase with an unusual module architecture which defines the new glycoside hydrolase family GH148

Fraction

Vol [mL]

Protein [mg/mL]

Spec. Activity [U/mg]

Purification factor

Yield [%]

Crude extract

25

8.5

2

1

100

Heat treated crude extract

22

0.9

8.3

4.2

41

Elution Source 15S

3

0.2

132.9

67.5

19.8