Table 1 Purification of recombinant EngU. Activity was measured by the DNS assay with 0.5% barley β-glucan as the substrate in 50 mM McIlvaine buffer pH 6.0 after incubation for 10 min at 80 °C.
Fraction | Vol [mL] | Protein [mg/mL] | Spec. Activity [U/mg] | Purification factor | Yield [%] |
---|---|---|---|---|---|
Crude extract | 25 | 8.5 | 2 | 1 | 100 |
Heat treated crude extract | 22 | 0.9 | 8.3 | 4.2 | 41 |
Elution Source 15S | 3 | 0.2 | 132.9 | 67.5 | 19.8 |