Table 3 Summary of the binding properties of purified EngUGST-CBM and GST (used as control) to soluble and insoluble substrates (see also Supplementary Figure 4).

From: A metagenome-derived thermostable β-glucanase with an unusual module architecture which defines the new glycoside hydrolase family GH148

Substrate

GST

 

EngUGST-CBM

 

Soluble substrates (gel shift assays)

r/r0

Kr

r/r0

Kr

β-glucan

1.03

>10

0.16

0.19

Lichenin

1.10

>10

0.30

0.43

CMC

1.15

>10

0.34

0.52

Laminarin

1.08

>10

1.07

>10

Insoluble substrates (batch assays)

Level of binding

 

GST

EngU GST-CBM

PASC

No binding

Strong

Avicel

No binding

Weak

CF1 cellulose

No binding

No binding

Xylan (birch wood)

No binding

Weak

Pachyman

No binding

No binding

Auxoferm

No binding

No binding

  1. Kr, retardation coefficient (mg × ml−1). r 0 and r, relative migration distance without and with substrate (at 1 mg × ml−1), respectively. The batch assays for insoluble substrates were performed with 100 µg protein and 50 mg substrate.
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