Table 4 Site-directed mutants and their relative activity compared to wild type EngU. Hydrolytic activities towards 2% carboxymethyl-cellulose (CMC) were determined using the DNS assay with heat-treated, cleared preparations of EngU and mutants.

From: A metagenome-derived thermostable β-glucanase with an unusual module architecture which defines the new glycoside hydrolase family GH148

Protein

Relative CMCase activity

EngU wt

100%

EngU D123A

3%

EngU D151A

104%

EngU E183A

124%

EngU E235A

28%

EngU E239A

0%

EngU E581A

0%

EngU E593A

51%

EngU D605A

32%

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