Figure 1
From: A simple high throughput assay to evaluate water consumption in the fruit fly
Water consumption assay. (a) Schematic diagram of assay. (b) Water consumption using non-dehydrated and dehydrated food. (c) To facilitate visualisation of consumed water, blue dye has been added to the water and can be seen in the abdomen of the fly. (d) The number of flies per chamber does not influence individual water consumption. Five, ten, fifteen, or twenty files were tested per assay for 3 days. (e) Temperature regulates water consumption in flies. The water consumption assay was tested in either 18 °C or 25 °C conditions for 3 days. (f) Desiccation stimulates water consumption in flies. The water consumption assay was tested in either low humidity (55%) or high humidity (70%) conditions for 3 days. All data represented mean ± S.E.M (n = 3–6). Student’s t-test, **p < 0.01; ***p < 0.001; n.s., not significant.
