Figure 4

The dopamine receptor DopR1 regulates water consumption. (a) RNA Seq reads of DopR1 transcripts obtained from the heads of dehydrated or control flies. (b) DopR1 mRNA levels were analysed by RT-qPCR. (c) Water consumption of w ¹¹¹⁸ control and DopR1 mutant flies (dumb ²). (d) Food consumption of w ¹¹¹⁸ control and DopR1 mutant flies (dumb ²) highlights a specific defect in water consumption. (e) Proboscis extension response (PER) of w ¹¹¹⁸ control and DopR1 mutant flies to H₂O and 5% sucrose solution (f) L-dopa (3 mg/ml) pre-treatment increases the PER response to water in w ¹¹¹⁸ control but not DopR1 mutant flies. Sucrose responses are similar between treatments and genotypes indicating a water specific defect. For PER response, each independent trial consisted of ≥ 10 animals. (g) Water consumption of control (UAS-DopR1 RNAi/  +), elav-Gal4 > DopR1 RNAi and MB247-Gal4 > DopR1 RNAi flies. (h) Water consumption of control (w ¹¹¹⁸), DopR1 mutant (elav-Gal4/ + ; dumb ²/dumb ²) and rescue (elav-Gal4 > UAS-DopR1; dumb ²/dumb ² and MB247-Gal4 > UAS-DopR1; dumb ²/dumb ²). All data represented mean ± S.E.M (n = 3–6). Student’s t-test or one-way ANOVA followed by Tukey’s post hoc test, **p < 0.01, ***p < 0.001; n.s., not significant.