Figure 5
From: A simple high throughput assay to evaluate water consumption in the fruit fly
DopR1 is required for water taste. Extracellular bristle recordings of w 1118 control, ppk28 mutant (ppk28 Δ), DopR1 mutant (dumb 2 and elav-Gal4/ + ;;dumb 2/dumb 2) and rescue (elav-Gal4 > UAS-DopR1; dumb 2/dumb 2 and MB247-Gal4 > UAS-DopR1; dumb 2/dumb 2) flies after stimulation with water or 40 mM sucrose. (a) Representative spikes showing response to water (1 mM KCl) or 40 mM sucrose. (b,c) Mean total spike number for the first second of response to (b) water or (c) sucrose is shown. 3 to 5 L-type labellar bristles were recorded per animal. All data represent mean ± S.E.M (n = 9–17 animals). One-way ANOVA followed by Tukey’s post hoc test, **p < 0.01, ***p < 0.001; n.s., not significant.
