Figure 2
Inhibition of EMCN mRNA and protein levels in vivo after intravitreal injection. EMCN and control siRNA were injected directly into the vitreous humor of a P4 mouse eye and inhibition of EMCN levels were assessed by (a,b) qRT-PCR and (c) immunofluorescence. (a) Knockdown of EMCN mRNA levels was evaluated at 48 hr after injection using different concentrations of siEMCN compared to control. (b) Inhibition of EMCN mRNA was assessed at different time points post injection using 10 pmol of EMCN siRNA. (c) Flat-mounted images of isolectin B4 (green) and EMCN (red) stained retinas from P6 neonatal mice 48 hr post intravitreal injection of control or siEMCN (10 pmol) shows knockdown of EMCN expression. *P < 0.05, **P < 0.01, siEMCN vs siCtrl. Scale bar, 100 μm.
