Figure 7
EMCN expression modulates ECM-induced tube morphogenesis. Representative 6 hr images of HRECs transfected for 24 hr with (a) siCtrl or (b) siEMCN or infected with (d) AdGFP or (e) AdEMCN plated on polymerized basement membrane extract (BME) in the presence of VEGF (10 ng/mL) are shown. (c,f) Quantification of total tube length 12 hr after plating on BME in the presence and absence of VEGF shows (c) knockdown of EMCN expression inhibits ECM-induced tube formation while (f) over-expression of EMCN enhances ECM-induced tube formation. *P < 0.05, ***P < 0.001 siEMCN vs siCtrl or AdEMCN vs AdGFP; *P < 0.05, **P < 0.01 VEGF vs no VEGF. Scale bar 100 μm.
