Figure 4 | Scientific Reports

Figure 4

From: Efficiency of novel nanocombinations of bovine milk proteins (lactoperoxidase and lactoferrin) for combating different human cancer cell lines

Figure 4

Investigation of the apoptotic effect of the most effective NPs using double nuclear staining. (a) Flow charts of annexin-PI analysis of cancer cells lines (I) including fibroblast cells, Caco-2 cells, HepG-2 cells, MCF-7 cells and PC-3 cells following 72 h treatment with (II) LPO-loaded NPs, (III) LF-coated NPs, (IV) LPO + LF-loaded NPs, (V) LF coated LPO-loaded NPs and (VI) 5-FU. (b) The percentage of total apoptosis in NPs-treated normal and cancer cells in comparison with untreated cells. (c) Fluorescence images of ethidium bromide-acridine orange staining of cancer cells lines (I) including Caco-2 cells, HepG-2 cells, MCF-7 cells and PC-3 cells following 72 h treatment with (II) LPO + LF-loaded NPs, (III) LF coated LPO-loaded NPs and (IV) 5-FU.

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