Figure 4

The effect of Sqle on ROS scavengers. (A–C) C2C12 cells were cultured and transfected as mentioned in Fig. 2. Cells were prepared on 2 d of myogenic induction and subjected to RT-qPCR. Gene expression levels (A;Sod, B;Gpx1, C;Cat) were analysed using the 2^−∆∆Cq method. Gapdh was used as an internal control gene. The fold change of ROS scavengers was calculated by dividing expression levels in the myogenic group by those of control group. The experiment was done at least three times repeats. The significant differences were shown as *P < 0.05 versus control.