Figure 1
From: Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma
IL-20 expression in HCC tumors was correlated with overall survival, local recurrence free survival, and cyclin D1 expression. (a) Expression of IL-20 in 104 HCC tissues were determined using IHC staining and classified as IL-20 low expression (IL-20 low Exp.) and high IL-20 high expression (IL-20 high Exp.) (400X). The expression levels of IL-20 in tissue samples were analyzed using H scoring and graded as low IL-20 expression (IL-20-L, H < medium, n = 52) and high IL-20 expression (IL-20-H, H > medium, n = 52). Kaplan-Meier plots were used to predict (b) local recurrence free survival and (c) overall survival based on IL-20 expression levels. (d) The mRNA expression of IL-20 in non-tumor tissue and tumor tissue. Quantification analysis of mRNA was normalized with GAPDH. Values are means ± SD. Data are representative of three independent experiments. (e) The correlation of IL-20 and cyclin D1 mRNA expression in 26 patients with HCC. Values represent log10 of mRNA expression change in each individual patient. Each point represents each individual. (f) IL-20 and cyclin D1 mRNA expression in human hepatoma cell lines HepG2, Hep3B, and Huh7 were analyzed using qRT-PCR with specific primers.
