Figure 2
From: Anti-IL-20 monoclonal antibody inhibited tumor growth in hepatocellular carcinoma
Cell migration, TNF-α expression, and MMP-13 mRNA expression were higher in IL-20-treated ML-1 cells. (a,b) Cell migration was evaluated using a real time migration assay. ML-1 cells were treated with IL-20 (200 ng/ml), 7E (2 μg/ml), or IL-20 (200 ng/ml) plus 7E (2 μg/ml) for 16 h and monitored. (a) Representative time-lapse images for tracking motion distance of ML-1 cells are shown for each group. The motion distance of each cell is presented in different colors (count = 15 cells). (b) Quantization of the motion distance (in μm) of ML-1 cells (count = 15 cells). *P < 0.05 compared with the untreated group, # P < 0.05 compared with the IL-20 treated group, each done in quadruplicate. Values are means ± SD. Data are representative of three independent experiments. ML-1 cells were treated with IL-20 (200 ng/ml), 7E (2 μg/ml), or IL-20 (200 ng/ml) plus 7E (2 μg/ml) for 6 h, and the expression levels of TNF-α (c) and MMP-13 (d) were analyzed using qRT-PCR with specific primers. Quantification analysis of mRNA was normalized with GAPDH. *P < 0.05 compared with the untreated group, # P < 0.05 compared with the IL-20-treated group. Values are means ± SD. Data are representative of three independent experiments.
