Figure 3
From: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
Changes in mutation frequency following selection. (a) Mutation frequencies from the initial population plotted against mutation frequencies from negative selection presented as log2 transformed frequencies. Shades of red indicate adjusted P values as determined by Fisher’s exact test adjusted using the Benjamini-Hochberg procedure. Stop codons with significantly altered frequencies are colored in black. (b) Mutation frequencies from negative selection plotted against positive selection mutation frequencies, log2 transformed. P values are calculated as in (a).
