Figure 6
From: Deep mutational scanning of S. pyogenes Cas9 reveals important functional domains
Specificity enhancing mutations map to the PI domain. Cartoon structure of the PI domain of SpCas9 bound to dsDNA based on PDB: 5f9r (ref.27). Residues that were found to have mutations which were enriched in both positive and negative selection are shown as red spheres. The PAM nucleotides are colored in pale blue.
